Contact Info

Adam P. Hitchcock

Canada Research Chair

in Materials Research
CLS-CCRS
B.I.M.R
McMaster University
Hamilton, ON
Canada L8S 4M1
V: +1 905 525-9140
    x24729
F: +1 905 521-2773
E: aph@mcmaster.ca
U: unicorn.mcmaster.ca
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Visualising proteins on polymer surfaces under water

WHO:  Adam Hitchcock, Cynthia Morin, BIMR, McMaster University

WHERE:  Advanced Light Source  BL 7.0  Scanning Transmission X-ray Microscope (STXM)

WHEN:   October 2000                        POSTED:  20 Mar 2001
 
WHAT:  Biomaterials are the basis of technologies which interface artificial structures with living organisms. An important issue in this field is biocompatibility, which is often linked to specificity in first interactions of a material with particular proteins. Preferrential attachment of specific proteins to individual components of surface phase segregated polymers is believed to be a critical aspect of biocompatibility. We are using Scanning Transmission X-Ray Microscopy (STXM) to study this problem. Figure 1 is a false-color composite image showing how X-ray microscopy can be used to map the distribution of thin layers of fibrinogen (a key blood protein) relative to two filler particle components - StyreneAcrylonitrile (SAN) and poly-isocyanate poly-addition product (pipa) in a model polyurethane (supplied courtesy of Dow Chemical). The sample is prepared by depositing fibrinogen from a dilute buffer solution and was measured under 5-10 micron of water. The quantitative color coded chemical map was derived by pixel-by-pixel fits of C 1s absoprtion spectra to reference spectra of the pure material.

 

 

 

 

 

 

 

 

Figure 2 plots the fit to the spectrum extracted from the blue pixels where the fibrinogen is most dense. Other work has shown that STXM is capable of detecting adsorbed protein at the monolayer level.

WANT FURTHER INFORMATION ? CONTACT Adam Hitchcock

References: J. Synchrotron Radiation 8 (2001) 66;

                  J. Biomaterials Science: Polymers (2002) in press.

Credits: Polymer sample courtesy of Gary Mitchell (Dow Chemical). Work carried out at BL 7.0.1 STXM by Cynthia Morin, Tolek Tyliszczak, Hiromi Ikeura-Sekiguchi and Adam Hitchcock. Curve fit carried out using conjugate gradient optimization, courtesy of Bill Loo (Stanford). Research supported by NSERC (Canada) and the Canada Research Chair program.

2002 A.P. Hitchcock / McMaster University - All Rights Reserved
web site by Christopher Amis. Last updated on 04/23/2002 (aph)