WHO:  Adam Hitchcock, Göran Johansson, James J. Dynes, BIMR, McMaster University;  
             George Swerhone, John Lawrence, NWRI, Saskatoon
             Tolek Tyliszczak, Advanced Light Source, Berkeley Lab, Berkeley, CA 94720 USA
 
WHERE:  Advanced Light Source  BL 5.3.2  Scanning Transmission X-ray Microscope (STXM)
 
WHEN:   28 January 2006                POSTED:  10 April 2006
 
WHAT:  An apparatus to allow rotation of samples at the focus of the X-ray beam in scanning transmission X-ray microscopy has been developed and used to measure three-dimensional chemical distributions with sub 100 nm spatial resolution. The movie shows an example of the rotation sequence for a wet biofilm enclosed in the tip region of a thin-walled glass capillary. The figure (click for full size) shows examples of the protein structure derived from fits of a data set consisting of a series of 12-energy image sequences (529-534 eV) measured at 36-angle (0-180 deg in 5 deg intervals). The protein is one of five chemical component maps generated by fitting the 4-d data volume to reference spectra of {water, SiO2, protein, lipid and polysaccharide}. This energy regime is in the O 1s absorption region just below onset of the strong absorption by the water and glass.

REFERENCE: Extended abstract for Microscopy & Microanalysis 2006 (Chicago, July 28-Aug 2, 2006)

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