WHO: Adam
Hitchcock, G�ran Johansson, James J. Dynes, BIMR, McMaster University;
George
Swerhone, John Lawrence, NWRI, Saskatoon
Tolek
Tyliszczak, Advanced Light Source, Berkeley Lab, Berkeley, CA 94720
USA
WHERE: Advanced Light
Source BL 5.3.2
Scanning Transmission X-ray Microscope (STXM)
WHEN: 28 January 2006 POSTED:
10 April 2006

WHAT: An apparatus to allow rotation of samples at
the focus of the X-ray beam in scanning transmission X-ray microscopy
has been developed and used to measure three-dimensional chemical
distributions with sub 100 nm spatial resolution. The movie
shows an example of the rotation sequence for a wet biofilm enclosed
in the tip region of a thin-walled glass capillary. The figure
(click for full size) shows examples of the protein structure derived
from fits of a data set consisting of a series of 12-energy image
sequences (529-534 eV) measured at 36-angle (0-180 deg in 5 deg
intervals). The protein is one of five chemical component maps generated
by fitting the 4-d data volume to reference spectra of {water, SiO2,
protein, lipid and polysaccharide}. This energy regime is in the
O 1s absorption region just below onset of the strong absorption
by the water and glass.
REFERENCE: Extended abstract
for Microscopy & Microanalysis 2006 (Chicago, July 28-Aug
2, 2006)
WANT FURTHER INFORMATION ? Contact Adam
Hitchcock